Use one Xport Bio Plate for every 1,250 U.S. gallons (4,750 L) of system capacity, more for heavy bio loads. Rinse material well with aquarium water or purified tap water. For superior results, seed the material usingFlorinbacter7, Microbacter Start XLF, Microbacter7, or Florinbacter Clean as appropriate for your aquarium, by placing Xport Bio Plate in a plastic container, filling it with aquarium water, and then adding the full recommended dose of either bacterial product, based on the size of your aquarium, to that container. Allow to stand overnight and then add the seeded Xport Bio Plate to your system. Discard soak water. It is recommended that you use sponge, fine filter floss or other mechanical filtration ahead of Xport Bio Plate to prevent clogging with organic material. For maximum results, also provide a good carbon source for growing bacteria, such as Brightwell FlorinAxis as directed on its label. Caution: Keep out of reach of children. Not for human use or consumption.